Permeation of human ovarian tissue with cryoprotective agents in preparation for cryopreservation

Optimal vitrification protocol for mouse ovarian tissue cryopreservation: effect of cryoprotective agents and in vitro culture on vitrified-warmed ovarian tissue survival.

STUDY QUESTION What is the optimal vitrification protocol according to the cryoprotective agent (CPA) for ovarian tissue (OT) cryopreservation? SUMMARY ANSWER The two-step protocol with 7.5% ethylene glycol (EG) and 7.5% dimethyl sulfoxide (DMSO) for 10 min then 20% EG, 20% DMSO and 0.5 M sucrose for 5 min showed the best results in mouse OT vitrification. WHAT IS KNOWN ALREADY Establishing...

Human Ovarian Tissue Cryopreservation as Fertility Reserve

I-10: Vitrification of Baboon Ovarian Tissue:Will Vitrification Replace Conventional Freezing for Ovarian Tissue Cryopreservation?

Background: The aim of this study was to evaluate the efficacy of a vitrification protocol developed by our group to cryopreserve ovarian tissue. For this, we used ovaries from non-human primates in order to have an animal model close to the clinical setting. Materials and Methods: Ovarian biopsies from five adult baboons were vitrified, warmed and autografted. After five months, follicle survi...

Indications for cryopreservation of ovarian tissue.

For patients who are planning to have chemotherapy, radiotherapy or to undergo bilateral oophorectomy, loss of ovarian function will result in premature ovarian menopause and loss of fertility. For these women, although there is no successful method for the cryopreservation of human oocytes, ovarian tissue cryobanking is proposed with a view to its autotransplantation at a later date or the iso...

Evaluation of Cryoprotective Agents for Use in the Cryopreservation of Equine Spermatozoa

The first equine pregnancy using sperm cryopreserved in glycerol was reported in 1957,1 and glycerol is still considered the optimal cryoprotectant for equine sperm.2–4 However, this opinion is largely based on data from other species, and research that evaluates additional cryoprotective agents (CPA’s) specifically for equine sperm is lacking. Other compounds such as ethylene glycol, dimethyl ...